Cannabidiol – CBD discovered in 1940. It is one of some 113 identified cannabinoids in cannabis plants and accounts for up to 40% of the plant’s extract – a non-psychoactive constituent of cannabis has been declared to induce neuroprotective impacts in different experimental forms of brain injury. We pursue investigating whether this drug could also improve locomotor recovery of rats submitted to spinal cord cryoinjury.
Rats were distributed into 5 experimental groups. Rats were offered to laminectomy in vertebral segment T10 followed or not by the utilization of liquid nitrogen for 5 seconds into the spinal cord at the very level to cause cryoinjury. The rats received injections of vehicle or Cannabidiol (20 mg/kg) immediately before, 3h after and daily for six days after surgery. The Basso, Bresnahan, and Beattie motor evaluation analysis was utilized to assess motor function post-lesion 1 day before surgery and on the 1st, 3rd, and 7th postoperative days. The extent of the injury was evaluated by hematoxylin-eosin histology and FosB expression. Cryogenic injury of the spinal cord resulted in a important motor deficit. Cannabidiol (CBD)-remedied mice exhibited a higher Basso, Bresnahan, and Beattie locomotor record at the end of the 1st week after spinal cord injury:
- Lesion + Vehicle
- Day 1: zero
- Day 7: four
- Lesion + Cannabidiol 20 mg/kg
- Day 1: zero
- Day 7: seven
Further, at this time there was a significant decrease in the extent of tissue damage and FosB expression in the ventral horn of the spinal cord. The actual research confirmed that the utilization of liquid nitrogen to the spinal cord induces quantifiable and reproducible spinal cord injury associated with locomotor function impairments. Cannabidiol (CBD) improved locomotor functional recovery and reduced injury extent, suggesting that it could be useful in the treatment of spinal cord lesions.